Registration of blood preparations
infection using the non-contact method

For the first time the existence of weak intrinsic cells emission in cells of animals and plants which induces division of surrounding cells (in particular in ultra-violet area of a spectrum) was described by Gurvich A.G. [1]. He named this phenomenon "mitogenetic rays". The nature of the chemical reactions bringing about luminescence is still not clear.

After the publication of the first works concerning intrinsic superweak chemiluminescence of cells, tissues and blood preparations, numerous attempts were made to use this parameter with a view of clinical diagnostics. The parameter proved to be significantly different when a number of pathologies were observed. The measurement of not activated chemiluminescence in clinical medicine has not become a common practice yet, while the measurement of chemiluminescence with activators has became quite customary [2].

On the other hand, judging by the experiments [3-7] and calculations [7] weak intrinsic emission of nonequilibrium thermodynamic systems is not that small. Its registration during electrolysis of solutions and biochemical reactions is made possible by using either the noncontact method (based on changes of oxidation-reduction potential of water solutions), or spectral methods [8, 9]. Hence, there was an idea to use the results of works [3-9] for clinical diagnostics. To this end an experiment was carried out consisting in artificial infection of blood preparations with microbes Lactobacillus plantarum.

The experiment scheme (12/15/2005). The standard polypropylene test tubes (10 ml, thickness of glass ~0.9 mm, hermetically plugged) were filled in sterile conditions with newly made aseptic dredge of human blood cells elements (V=4,5 ml), restored by adding a physiological erythrocytes mass solution (2:1). Then suspension of erythrocytes was artificially infected with Lactobacillus plantarum in quantity 8x105 and 16x105 cells per test tube. We observed the influence of a non-equilibrium system on the process of microorganisms reproduction and dynamics of blood preparations (i.e. influence of newly made neutral anolyte passed through cathode (АNC). A dose of of ANC-0.4 ml (concentration of active chlorine 500 mg/l) was added in test tubes in conditions identical to those of the experiment. All the test tubes were placed into standard plastic syringes "Luer" (V 20 ml). The space between a test tube and a syringe was filled with distilled water (V = 8 ml) and pressurized by a rubber lining. Syringes were placed in a thermostat at T + 37o C. Water рН and redox potential (comparing with silver-chlorine electrode and with distilled water in a syringe containing empty inner test tube) were measured every 24 hours using the device рН-150.

Fig. 1. Schedules of noncontact change of distilled water redox potential in comparison with redox potential of standard type regarding influence of newly made suspension of erythrocytes 1-3: a) with addition of anolyte ~(10:1), b) without any additions; 2 (3) - with artificial infection with microbes Lactobacillus plantarum ~4x105, (2x105) per ml.

The results of the experiment are presented in figure 1. Each point on the schedule presents an average value of 5 individual tests + - a standard deviation. During the experiment we noticed an insignificant change of reference template water from +308 millivolts up to +260 millivolts (comparing to a silver-chlorine electrode) and insignificant changes of reference template, experiment template and standard template рН (~0,3 units).

Thus, live systems (just as newly made blood preparations and microorganisms) actively interact with each other and become a source of electromagnetic emission. During this processes one can observe noncontact activation of liquids (NCAL) caused by supercoherent electromagnetic emission of resonant microclusters [7, 8]. Discovery of NCAL phenomenon in live systems can become a basis for new and very simple supersensitive noncontact methods of live systems monitoring without direct intervention into its structure and new methods of diagnostics in medicine, microbiology and in other branches of science.

References:
1. Gurvich A.G. Mitogenetic radiation, Moscow, Gosmedizdat, 1934.
2. Vladimirov Y.A. Lectures ‘Physical-chemical foundations of cell pathology’ http://www.biophysics.hotmail.ru/files/doc/doc.htm.
3. Prilutskij V.I., Bakhir V.M. ‘Electrochemically activated water: anomalous properties, the mechanism of biological activity’ Moscow, VNIIIMT AO NPO ‘Screen’, 1997 p. 228 sb10-1.htm.
4. Shironosov V.G., Shironosov E.V. ‘Experiments on noncontact activation of water’// ‘Electrochemical activation in medicine, farming, industry’. The second international symposium. Lectures and abstracts. Moscow, VNIIIMT, 1999, part 1, p.66 sb15-12.htm.
5. Dubrovskaya O.A., Mulakhmetov R.F., Shironosov V.G. ‘Phenomenon of noncontact activation during chemical reactions, with microhydrin as a source of activation’ Collection of abstracts of VNKSF-8, Ekaterinburg, 2002, p.597-599 sb26-2.htm.
6. Karatayeva S.Y., Shironosov V.G. ‘Phenomenon of noncontact activation of liquids by living organisms. Collection of reports abstracts of VNKSF-10, Moscow, 2004, p. 825-827 sb33-3.zip.
7. Shironosov V.G. ‘Resonance in physics, chemistry and biology’ Izhevsk, Publishing house “Udmurt University’, 2001, p. 92, sb22.htm
8. Konovalova N.A., Menshikova S.G., Shironosov V.G. ‘Registration of phenomenon of noncontact activation of liquids using methods of microwave spectroscopy’ Collection of abstracts of the eighth Russian scientific conference of students of the departments of physics, Ekaterinburg, 2002, p. 590-592, sb26-1.htm.
9. Berdova E.S., Glukhova N.A., Shironosov V.G., Kurganovich V.S. “Phenomenon of changes of potassium permanganat spectrum during noncontact activation. Collection of abstracts of VNKSF-10, Moscow, 2004, p.543-544, sb33-1.zip.
10. Shironosov V.G. ‘Phenomenon of alive water and its simple explanation’. Collection of abstracts of the thirteenth international symposium ‘2003- International year of water’, Austria, 2003. p.246-249, sb28-1-3.htm.