"IIS-RT" - 1997. Collection № 33-2

Non-contact influence of electrochemically activated water solutions on erythrocyte sedimentation dynamics

Kazankin D. S., Shironosov V. G.
Scientific Research Center "Resonant Technologies" Udmurt State University, http://v4.udsu.ru/science/untsrt
Scientific Research Center "IKAR", ikar.udm.ru, ikar@udm.ru
Collection of abstracts of VNKSF-10, Moscow, 2004, p. 822-824

 

Presently influence of various factors on water component of alive systems arouses interest of scientists. One of the study directions is the influence of non-contact effect of electrochemically activated water solutions on erythrocyte sedimentation rate.

There are number of ways to activate water environments: mechanical, thermal, acoustic, magnetic, electric, etc. We used a new perspective method based on theoretically (1984) and experimentally (1999) [1] proved effect of non-contact liquid activation. This method is applied using a membraneless elecrtrolyzer. The phenomenon of non-contact liquids activation (NCLA) consists in the change of water solutions physical parameters while their chemistry remains unchanged. Activation was performed in hermetic containers made of dielectric materials (such as plastic, polythene, fluoroplastic, etc). The containers were placed in electrochemically activated environments (ECA) (i.e. in water environment where electrolysis is or was carried out). The non-contact activated solutions, produced in such a way and being in an unstable thermodynamic state, present an issue of interest in terms of their influence on biological systems.

Whole human blood, treated to prevent its coagulation was used as a model of biosystem. It is necessary to take into account that blood is not just a colloid solution, but a biological tissue taken in conditions of stress. Subsidence of blood cells reflects a reaction of a living tissue to a complex of external influences. From this perspective the phenomenon of blood erythrocytes subsidence was adeptly called "erythrocyte sedimentation rate" (ESR) within several decades.

Two series of the experiments were performed to study influence of the ECA environments on blood and its components. During the first series human erythrocytes separated from plasma (V=150 ml) were kept for a long period of time in standard plastic packages for blood transfusion at t = +2 оС. During the experiment a package containing packed red blood cells was placed in a container filled with 0,9 % water solution of NaCl, V = 3,5 l. In this experiment package electrolysis at U = 4 volts, I = 500 ma was constantly maintained. In the control package electrolysis was not performed, other things being equal. By observing the results, erythrocytes charge decreases during the experiment. This process becomes apparent due to acceleration of erythrocytes subsidence. Reliable distinctions were observed on the 5th day of experiment. The maximum difference was registered on the 25th day and amounted to 7 mm per day.

The second series of the experiments was carried out to prove the ability of ECA environments to perform non-contact action after a long and continuous process of electrolysis. Hermetic containers made of latex (thickness – 0.7 mm) were filled with samples of whole blood (V = 1 ml) and placed into ECA environment received as the result of 1150 hours long electrolysis (0.9 % NaCl solution; parameters, conditions and reference template identical to those used during the first series of experiment). The exposure of the samples lasted about 2 hours. Then blood was placed into standard capillaries for ESR measurement. ESR increases during non-contact ECA environment action. Differences amounted to 73 %. The typical schedule is presented in fig.

Fig. Erythrocyte sedimentation dynamics: l - border shift (mm),

1 - experiment test, 2 - control test

During the experiment we observed the emission of electrical excitation energy from the area adjoining to electrodes through water environment and polymers. Due to this energy redox potential of the water solution placed into hermetic container shifts to negative range during non-contact activation, while chemistry of a solution remains unchaged. Molecular mechanisms of this phenomenon are identical to those of a living cell. In both cases origin and transfer of electrical-excited states are based on ability of water molecules included in water clusters to perform homolytical dissociation. We should take into consideration influence of low energy density (0,5 eV; hydrolysis of ATP molecule), whereas 5 eV is needed to break of H-O bond of a water molecule. ROS ware formed as a result of water dissociation. ROS can cause lipid peroxidation in erythrocytes membranes during free radical reactions. The possibility of using EES for producing ROS was convincingly proved in the last researches [2]. Lipid peroxidation provokes membranes degradation and erythrocytes surface charge decrease, and this causes increase of erythrocytes subsidence speed.

References:
[1] Shironosov V.G., Shironosov E.V., Experiments on non-contact electrochemical activation of water, Collection of abstracts of the 2nd international symposium "Electrochemical activation in medicine, farming and industry", Moscow, VNIIIMT NPO "Screen ", part 1, p.66-68 (1999) - sb15-12e.htm.

[2] Baskakov I.V., Voejkov V.L., Role of the electronic-excited states in biochemical processes. Biochemistry, volume 61. №7, p. 1169-1181, 1996.